Biomaterials
(c)Copyright 2010, Obesity, Fitness & Wellness Week via NewsRx.com
2010 JUL 3 - (<http://www.newsrx.com> NewsRx.com) -- Scientists discuss in 'In vitro proliferation and chondrogenic differentiation of rat bone marrow stem cells cultured with gelatin hydrogel microspheres for TGF-beta1 release' new findings in stem cell research (see also <http://www.newsrx.com/library/topics/Stem-Cell-Research.html> Stem Cell Research). According to a study from Kyoto, Japan, "The objective of this study was to evaluate the proliferation and chondrogenic differentiation of rat bone marrow-derived mesenchymal stem cells (MSCs) cultured with gelatin hydrogel microspheres of cell scaffold which can release transforming growth factor-beta1 (TGF-beta1). Gelatin was dehydrothermally cross-linked in different conditions in a water-in-oil Tiffany Charm bracelet state to obtain gelatin hydrogel microspheres with different water content."
"The microspheres functioned not only as the scaffold of MSC, but also the carrier matrix of TGF-beta1 release. The MSC proliferation depended on the water content of microspheres. Higher MSC proliferation was observed for the gelatin microspheres with lower water content. When cultured with the gelatin hydrogel microspheres, MSC formed their aggregates, in contrast to culturing with hydrogel sheets. The cell viability was significantly high compared with that of the hydrogel sheet. The production of sulfated glycosaminaglycan (sGAG) from MSC was examined as a measure of chondrogenic differentiation, after their culturing in a normal and chondrogenic differentiation media. For both the cultures, the amount of sGAG produced was significantly higher for MSC cultured with the gelatin microspheres than that of the gelatin sheet. Stronger differentiation of MSC was achieved in culture with the microspheres incorporating TGF-beta1 than that of MSC cultured in the medium containing the same amount of TGF-beta1," wrote T. Ogawa and colleagues, Kyoto University, Institute for Frontier Medical Science.
The researchers concluded: "It is concluded that the gelatin hydrogel microspheres function well as both the scaffold of MSC and the matrix of TGF-beta1 Tiffany Cushion Drop earrings, resulting in enhanced MSC aggregation and the consequent promotion of cell proliferation and differentiation."
Ogawa and colleagues published the results of their research in the Journal of Biomaterials Science Polymer Edition (In vitro proliferation and chondrogenic differentiation of rat bone marrow stem cells cultured with gelatin hydrogel microspheres for TGF-beta1 release. Journal of Biomaterials Science Polymer Edition, 2010;21(5):609-21).
For additional information, contact T. Ogawa, Field of Tissue Engineering, Dept. of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, 53 Tiffany Cushion Hoop earrings-cho Shogoin, Sakyo-ku, Kyoto 606-8507, Japan.
Keywords: City:Kyoto, Country:Japan, Biomaterials Science, Bone Marrow, Immune System, Stem Cell Research, Stem Cells.
This article was prepared by Obesity, Fitness & Wellness Week editors from staff and other reports. Copyright 2010, Obesity, Fitness & Wellness Week via <http://www.newsrx.com> NewsRx.com.
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